mTOR Prevents Ferroptosis in Cardiomyocytes

What differentiates ferroptosis in the heart from apoptosis and necrosis? Listen as Deputy Editor Merry Lindsey (University of Mississippi Medical Center) interviews lead author Takashi Matsui (University of Hawai'i) and content expert Lorrie Kirshenbaum (St. Boniface General Hospital Research Centre, Canada) about the exciting new study by Baba et al, one of the first to show that ferroptosis is distinct from autophagy, apoptosis and necrosis in cardiomyocytes. Ferroptosis is cell death characterized by excessive levels of iron and to iron-mediated reactive oxygen species generation. Did the authors find that glutathione peroxidase 4 (GPX4) played a role in cardiomyocyte ferroptosis? What connection did the authors find between the mTor pathway and iron-mediated cell death in cardiomyocytes? Listen, read, and view the beautiful cardiomyocyte isolation images to learn more.

 

Yuichi Baba, Jason K Higa, Briana K Shimada, Kate M. Horiuchi, Tomohiro Suhara, Motoi Kobayashi, Jonathan D. Woo, Hiroko Aoyagi, Karra S Marh, Hiroaki Kitaoka, and Takashi Matsui Protective Effects of the Mechanistic Target of Rapamycin against Excess Iron and Ferroptosis in Cardiomyocytes Am J Physiol Heart Circ Physiol, published November 10, 2017. DOI: 10.1152/ajpheart.00452.2017

Guidelines on Antibody Use in Physiology Studies

It is nearly impossible to avoid using antibodies in biological research, but are cardiovascular researchers validating reagents the right way? In our latest podcast on Guidelines in Cardiovascular Research, Editor in Chief Irving H. Zucker (University of Nebraska Medical Center) interviews authors Heddwen L. Brooks (University of Arizona) and Deputy Editor Merry L. Lindsey (University of Mississippi Medical Center), along with expert John Hollander (West Virginia University School of Medicine) about how best to advise researchers on antibody use in immunohistochemistry and immunoblotting for evaluating expression of specific proteins in the cells and tissues of interest. Given the ongoing call for increased rigor and reproducibility in scientific research, this Guidelines article podcast offers investigators a roadmap to what controls should be performed and what information should be recorded when using antibodies in cardiovascular physiology research. Listen now.

 

Heddwen L Brooks and Merry L. Lindsey Guidelines for Authors and Reviewers on Antibody Use in Physiology Studies Am J Physiol Heart Circ Physiol, published January 5, 2018. DOI: 10.1152/ajpheart.00512.2017