Archive for January 2021

Membrane Proteomic Profiling of the Heart

What are the latest technological advances for studying cell membrane proteins? Podcast host Lisandra de Castro Brás (East Carolina University) interviews lead author Tony Gramolini (University of Toronto) and expert Sarah Parker (Cedars-Sinai Medical Center) about the latest Review by Lee et al, which outlines challenges in the development of heart disease therapies related to the limited number of functional target proteins in cell surface receptors. The authors also examine potential limitations and workarounds for analyzing hydrophobic membrane proteins, discuss the importance of spatial location during sample harvesting, and review the potential of mass spectrometry tools for generation of robust and ample data. Our experts discuss a wide array of cutting-edge proteomic analysis tools, including tissue cytof, single-cell proteomics, and mass spectrometry, and give special attention to techniques for interrogating low-abundant proteins. This leads to an in-depth exchange about data reporting in research articles. What do the authors think is on the horizon for advanced interrogation of post-translational modifications and integrated AI-based analysis of the vast amounts of data produced by proteomic profiling? Listen to find out.

 

Shin-Haw Lee, Da Hye Kim, Uros Kuzmanov, Anthony O. Gramolini Membrane proteomic profiling of the heart: past, present, and future Am J Physiol Heart Circ Physiol, published January 15, 2021. DOI: doi.org/10.1152/ajpheart.00659.2020

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Aerosolized Nicotine and Cardiovascular Control

What are the acute effects of inhaling nicotine through an e-cigarette on cardiovascular neural regulation? In this episode Guest Editor Loren Wold (The Ohio State University) interviews lead author William Cooke (Michigan Technological University) and expert Ted Wagener (The Ohio State University) about the latest research by Gonzalez and Cooke on this timely topic. As more and more studies show that e-cigarettes do carry their own distinct health risks, Gonzalez and Cooke recruited naïve e-cigarette users, otherwise known as “never smokers,” for their e-cigarette study. The authors observed an acute increase in arterial pressure in study participants, which was associated with a blunting of peripheral sympathetic activity. Why is this important? The authors believe that there could be long term effects on the heart, including increases of heart rate and blood pressure, which could lead to pre-hypertension or chronic hypertension for non-smokers who opt to use electronic cigarette devices. Our experts unpack the implications of this unique study, the potential for researchers to separate effects of inhaled nicotine from effects of inhaled combustible tobacco toxicants, and what new federal guidelines may be forthcoming from the Food and Drug Administration (FDA) for e-cigarette devices in the coming year. Listen now to find out more.

 

Joshua Eric Gonzalez and William Harold Cooke Acute effects of electronic cigarettes on arterial pressure and peripheral sympathetic activity in young non-smokers  Am J Physiol Heart Circ Physiol, published November 8, 2020. DOI: doi.org/10.1152/ajpheart.00448.2020

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