Guidelines on Antibody Use in Physiology Studies

It is nearly impossible to avoid using antibodies in biological research, but are cardiovascular researchers validating reagents the right way? In our latest podcast on Guidelines in Cardiovascular Research, Editor in Chief Irving H. Zucker (University of Nebraska Medical Center) interviews authors Heddwen L. Brooks (University of Arizona) and Deputy Editor Merry L. Lindsey (University of Mississippi Medical Center), along with expert John Hollander (West Virginia University School of Medicine) about how best to advise researchers on antibody use in immunohistochemistry and immunoblotting for evaluating expression of specific proteins in the cells and tissues of interest. Given the ongoing call for increased rigor and reproducibility in scientific research, this Guidelines article podcast offers investigators a roadmap to what controls should be performed and what information should be recorded when using antibodies in cardiovascular physiology research. Listen now.

 

Heddwen L Brooks and Merry L. Lindsey Guidelines for Authors and Reviewers on Antibody Use in Physiology Studies Am J Physiol Heart Circ Physiol, published January 5, 2018. DOI: 10.1152/ajpheart.00512.2017