Behind the Bench Episode One: Cam Squared

Welcome to our very first episode of Behind the Bench from AJP-Heart and Circ, where we try to get the story behind the story from the researchers themselves. In this episode we talk with Camilla Wenceslau, an Assistant Professor at University of Toledo, and Cameron McCarthy, a Postdoctoral to Faculty Fellow also at University of Toledo. Camilla and Cam co-authored a recent article in AJP-Heart and Circ. They’re both early career investigators, and husband and wife. We enlisted the help of Jonathan Kirk (Loyola University Chicago) and Lisandra de Castro Bras (East Carolina University) to interview this dynamic duo, with special cameo appearances by some familiar AJP-Heart and Circ personalities. How did science bring “Cam Squared” together? Let’s find out.

 

Cameron G. McCarthy, Camilla F. Wenceslau, Fabiano B. Calmasini, Nicole S. Klee, Michael W. Brands, Bina Joe, and R. Clinton Webb Reconstitution of autophagy ameliorates vascular function and arterial stiffening in spontaneously hypertensive rats Am J Physiol Heart Circ Physiol, published October 23, 2019. DOI: doi.org/10.1152/ajpheart.00227.2019

Cortical Bone Derived Stem-Cell Therapy Reduces Apoptosis

How do cortical bone derived stem cells reduce scar size and improve cardiac function following myocardial infarction? Guest Editor Lorrie Kirshenbaum (St. Boniface General Hospital Research Centre) interviews authors Steven Houser (Temple University) and Alexander Hobby (Temple University), along with Associate Editor Junichi Sadoshima (Rutgers New Jersey Medical School), about the innovative new study by Hobby et al showing CBSCs reduce apoptosis of both myocytes and non-myocytes in a large animal model of reperfusion injury. Hobby et al found that CBSCs increased the number of T cells and macrophages in the heart, which may mean that the cardioprotective effects of CBSCs could be mediated through T cells. What do our experts have to say about the overall state of stem cell therapy, how CBSCs differ from other conventional types of stem cells, and the potential clinical application of CBSCs for post-MI wound healing? Listen now.

 

Alexander R. H. Hobby, Thomas E. Sharp 3rd, Remus M. Berretta, Giulia Borghetti, Eric Feldsott, Sadia Mohsin, Steven R. Houser Cortical bone-derived stem cell therapy reduces apoptosis after myocardial infarction Am J Physiol Heart Circ Physiol, published October 7, 2019. DOI: doi.org/10.1152/ajpheart.00144.2019

Angiogenic Exosomes from Vascular Progenitor Cells

Where did the search to find exosome donor cells lead the authors of the new study by Johnson et al? Editor in Chief Irving H. Zucker (University of Nebraska Medical Center) interviews authors Dong Liu (Morehouse School of Medicine) and Takerra Johnson (National Institutes of Health/ National Eye Institute), along with content expert Jean-Pyo Lee (Tulane University), about this novel study which utilized induced vascular progenitor cells as exosome donor cells to promote angiogenesis. The authors determined that secretion is more important to endothelial cells than differentiation, because secretion includes growth factors, cytokines, and exosomes. Compared to rat aortic endothelial cells, induced vascular progenitor cells have a greater secretion of exosomes. Johnson et al found that iVPCs promoted angiogenesis in a rat hindlimb ischemia model. What did the authors uncover when they investigated the microRNA cargo carried by iVPC exosomes? Listen and learn more.

 

Takerra K. Johnson, Lina Zhao, Dihan Zhu, Yang Wang, Yan Xiao, Babayewa Oguljahan, Xueying Zhao, Ward G. Kirlin, Liya Yin, William M. Chilian, and Dong Liu Exosomes derived from induced vascular progenitor cells promote angiogenesis in vitro and in an in vivo rat hindlimb ischemia model Am J Physiol Heart Circ Physiol, published October 1, 2019. DOI: doi.org/10.1152/ajpheart.00247.2019