Guidelines for Experimental Models of Myocardial Ischemia and Infarction

Myocardial infarction is a global health problem, and accordingly, treatment and prevention are the focus of a significant number of basic cardiovascular research projects. Animal models of myocardial ischemia, with or without reperfusion, are commonly used to study myocardial infarction, but with little guidance on best practices. Enter our new Guidelines in Cardiovascular Research article on “Guidelines for Experimental Models of Myocardial Ischemia and Infarction,” the result of a year-long collaboration of 20 internationally-recognized experts. Listen as Editor in Chief Irving H. Zucker (University of Nebraska Medical Center) interviews authors Merry Lindsey (University of Mississippi Medical Center), Gerd Heusch (Universitatsklinikum Essen), Karyn Przyklenk (Wayne State University School of Medicine), Elizabeth G. Murphy (NIH, National Heart, Lung, and Blood Institute), and Steven P. Jones (University of Louisville) about this comprehensive work establishing guidelines for rigor and reproducibility when using ischemia models. Strengths and limitations of a wide-range of specific models are analyzed, and the authors provide a veritable checklist for animal usage, study design, and experimental procedures. The authors also list data output details, in particular for in vivo studies, in easy-to-read table format. Listen as we unpack this landmark Guidelines in Cardiovascular Research article in this engaging conversation with key leaders in the field.

 

Merry L. Lindsey, Roberto Bolli, John M. Canty, Xiao-Jun Du, Nikolaos G. Frangogiannis, Stefan Frantz, Robert G. Gourdie, Jeffrey W. Holmes, Steven P. Jones, Robert Kloner, David J. Lefer, Ronglih Liao, Elizabeth Murphy, Peipei Ping, Karin Przyklenk, Fabio A. Recchia, Lisa Schwartz Longacre, Crystal May Ripplinger, Jennifer E Van Eyk, and Gerd Heusch Guidelines for Experimental Models of Myocardial Ischemia and Infarction Am J Physiol Heart Circ Physiol, published January 12, 2018. DOI: doi/10.1152/ajpheart.00335.2017

Guidelines for Measuring Cardiac Physiology in Mice

With increased focus on rigor and reproducibility in basic science, is now the best time to set the record straight on measuring cardiac physiology in mice? Yes, according to five experts who collaborated to write new comprehensive Guidelines in Cardiovascular Research on this vitally important topic. Listen as Consulting Editor David A. Kass (Johns Hopkins University School of Medicine) interviews co-authors Merry Lindsey (University of Mississippi Medical Center), Zamaneh Kassiri (University of Alberta, Canada), Jitka Virag (East Carolina University), Lisandra E. de Castro Bras (East Carolina University), and Marielle Scherrer-Crosbie (Hospital of the University of Pennsylvania). The authors discuss the strengths and limitations of various imaging modalities used to evaluate cardiac physiology, as well as their extensive literature analysis which led to the development of a checklist for authors and reviewers of minimum cardiac physiology information to be included in manuscript methods. In addition to echocardiography, echo Doppler, and MRI, what would these experts like to see developed for a “toolbox of the future”? Listen and find out.

 

Merry L. Lindsey, Zamaneh Kassiri, Jitka Amira Ismail Virag, Lisandra E. de Castro Bras, and Marielle Scherrer-Crosbie Guidelines for Measuring Cardiac Physiology in Mice Am J Physiol Heart Circ Physiol, published January 5, 2018. DOI: doi/10.1152/ajpheart.00339.2017

Temporal Dynamics of Acute and Chronic Heart Failure

What are the pathophysiological changes after myocardial infarction that effect the left ventricle, spleen, and kidney? Listen as Deputy Editor Merry Lindsey (University of Mississippi Medical Center) interviews lead author Ganesh Halade (University of Alabama at Birmingham) and content expert Zamaneh Kassiri (University of Alberta, Canada) about the novel study by Halade and co-authors which catalogued the time-dependent myocardial damage nexus to cardiosplenic and cardiorenal networks in heart failure pathology. The authors took a unique bedside-to-bench approach in their animal model study, inspired by the whole-body effects myocardial infarction patients experience, as MI triggers irreversible damage to the spleen and kidneys. With references to the new guidelines on ischemia and infarction models in animal experiments and to the push for increased rigor and reproducibility in basic science research, this podcast is a “must-listen” episode!

 

Ganesh V. Halade, Vasundhara Kain, and Kevin. A. Ingle Heart functional and structural compendium of cardiosplenic and cardiorenal networks in acute and chronic heart failure pathology Am J Physiol Heart Circ Physiol, published February 1, 2018. DOI: 10.1152/ajpheart.00528.2017